With three games left to play, and a Test series to be clinched, this week's two matches in Melbourne are critically important. The task begins with the game against the Melbourne Rebels at AAMI Park on Tuesday night before moving on to the vital second Test at the Etihad Stadium at the weekend.
Whereas Gatland quite rightly looked to protect his Test team in Canberra last weekend, sending out a back division that had barely met each other let alone practiced together, the Rebels will find themselves playing against some established tour combinations.
It is the 'Last Chance Saloon', if you like, for Test candidates to make a final impression ahead of selection for the second and third Tests and the players intend to make the most of their opportunity.
"We can't afford another loss because it will hurt the squad hugely. It's already hurt us and there were a lot of people upset throughout last week," explained Irish wing Simon Zebo.
"We were lucky to get the win on Saturday coming off a defeat like that, so we can't afford to let that happen again. We're all playing for places and we're playing to represent the Lions and you just don't want to be on the losing side. It's pretty vital we don't lose on Tuesday.
"It's not as easy as playing a weekend game, but you're still getting a chance to go out and represent the Lions, which isn't something you can take lightly. You would like to think there is always a chance of pushing for a Test sport and that if any player plays out of their skin, or has just one of those special days out on the pitch, then you obviously have a chance. "
That motivation alone should be enough to push the Lions to a victory over a Rebels side that will be led by Welsh international Gareth Delve and that will also feature the returning Qantas Wallabies scrum half Luke Burgess. This will be the biggest game in their short history and will give them a chance to capture a major scalp.
But their form throughout the Super Rugby season has been patchy, although recent home wins against the Stormers and Waratahs proved there is potential in head coach Damien Hill's outfit.
But there should be too much momentum on the Lions side of the fence for them to slip up again. The first Test win over the Wallabies in Brisbane, coupled with starts for four of the replacements from that game, should be enough for them to ride out any potential storm in Melbourne.