On the one side of the fence will sit a Qantas Wallabies team seeking to make the most of a once in a career opportunity, while on the other will be a Lions outfit desperate to end a 16 year famine since last tasting success in a series.
How do you read the runes? Will the Wallabies be undercooked after a three week camp in which their players have played no competitive rugby? Will the fact they haven't played a Test match in six months count against them?
Are the Lions ready for the step up to the pace and intensity of a Test match after five mediocre quality contests and a midweek defeat to the Brumbies with a makeshift side? So many questions are set to be answered in 80 minutes of compelling rugby at the Suncorp Stadium in Brisbane on Saturday night.
The series can't be won or lost on opening night, but both sides realise that whoever wins the first Test will hold a massive advantage moving into the next two games in Melbourne and Sydney.
In the 30 Test match series played by the Lions since 1891 there have only been four in which the winners of the first Test failed to win the series. Ironically, two of those have been the last two tours against the Wallabies.
In 1989, Finlay Calder's side had to come from behind after losing the first Test to complete a 2-1 victory, while John Eales' team did exactly the same 12 years ago after the Lions had given the home side a thumping in the first Test at The Gabba.
And that brings us to another fascinating fact about Saturday night's game: Suncorp Stadium is the Wallabies' favourite home venue, a ground at which they enjoy an 81% success rate, yet the Lions have won all seven Tests played in Brisbane since 1899.
Both teams will be looking to kick-off the series with a bang and the Wallabies will come into the game with three new caps, while the tourists will have eight new Test Lions in their starting line-up.
What might be critical for the Lions, however, is that there will be seven players with Test match experience in their side. Not one of the Wallabies has ever played against the Lions - or in a match where the visiting fans will be as vocal, if not more so, than their own supporters.
"Over the last two Saturdays, against the Reds and the Waratahs, a lot of the players from the Test side have been building momentum. We feel very positive about where we are," said Lions head coach Warren Gatland.
"Physically we feel like we're in good shape, the boys feel very fresh and they're looking forward to it. With the Australian team not having played for a few weeks, and with two or three players having been out injured, we need to really take it to them physically, and with the kind of tempo that they are used to putting on other sides."
Brian O'Driscoll will be playing in a Test match on a fourth successive tour, while Paul O'Connell will make it seven Tests in a row for the Lions when he packs down with Alun Wyn Jones in the second row. Their experience and leadership will be vital for new skipper Sam Warburton as he attempts to become the first Lions captain since Martin Johnson in South Africa in 1997 to win a series.
Both teams will be full of belief, but there will be a feeling of treading in the unknown because neither side has played against each other before and both are relatively new teams. Who will come out on top?
Go for the Lions because of their experience and battle-hardness. And the fact they never lose in Brisbane!