Christian Wade, who played for the Lions against the Brumbies after being summoned from Argentina, is one of six players stepping up into the 33-man squad. He is joined by lock David Attwood (Bath), flanker Matt Kvesic (Gloucester), No.8 Billy Vunipola (Saracens), centre Kyle Eastmond (Bath) and wing Marland Yarde (London Irish).
"This was the toughest EPS selection we've had to make with fierce competition for places in every area of the squad after an excellent summer for England players at all levels," said Lancaster.
"Twelve current England players were part of a victorious British & Irish Lions tour, eight others won their first caps in Argentina, a number of rested senior players will have benefited from their break and the future looks healthy after our Under 20 side won the Junior World Championship for the first time.
"Christian Wade and Marland Yarde brought their outstanding Aviva Premiership form to the tour, Kyle Eastmond showed the potential he has and by including him in the squad we get a chance to work with him some more as we build towards the QBE Internationals.
"Up front, David Attwood adds a physical presence to the second row and in Matt Kvesic and Billy Vunipola we have young back row players who will challenge our more established back row players hard."
Hooker Dylan Hartley is named in the squad and will return to action in September after an 11-week suspension following his sending off in the Aviva Premiership final. Hartley was one of the original picks for the Lions before his suspension ruled him out of the tour.
"I had a long hard think about it and a long conversation with him. I wanted to be reassured that he understands where the line is, what is right and what is wrong. He accepts that and he knows he needs a strong start to the season with Northampton as changes can be made with Rob Webber and David Paice pushing hard," said Lancaster.
"Unfortunately we can only have 33 in the Senior EPS but those who just missed out have plenty to offer. They are still very much in the international picture and there is flexibility built into the system that facilitates that."
There are first England Saxons call-ups for Jack Nowell (Exeter Chiefs), one of the 2013 Junior World Championship winners, and Luke Wallace (Harlequins) and the squad also includes the return of 2007 World Cup finalist Mathew Tait (Leicester Tigers).
Both squads will meet at Loughborough later this month for a three-day training camp and the senior squad reconvenes a fortnight before facing Australia on November 2. The Saxons are due to face the O2 Ireland Wolfhounds and Scotland A early in 2014.
ENGLAND SENIOR EPS (33)
David Attwood (Bath Rugby)
Dan Cole (Leicester Tigers)
Alex Corbisiero (Northampton Saints)
Tom Croft (Leicester Tigers)
Dylan Hartley (Northampton Saints)
Matt Kvesic (Gloucester Rugby)
Joe Launchbury (London Wasps)
Courtney Lawes (Northampton Saints)
Joe Marler (Harlequins)
Ben Morgan (Gloucester Rugby)
Geoff Parling (Leicester Tigers)
Chris Robshaw (Harlequins)
Billy Vunipola (Saracens)
Mako Vunipola (Saracens)
David Wilson (Bath Rugby)
Tom Wood (Northampton Saints)
Tom Youngs (Leicester Tigers)
Chris Ashton (Saracens)
Brad Barritt (Saracens)
Mike Brown (Harlequins)
Freddie Burns (Gloucester Rugby)
Danny Care (Harlequins)
Lee Dickson (Northampton Saints)
Owen Farrell (Saracens)
Toby Flood (Leicester Tigers)
Ben Foden (Northampton Saints)
Alex Goode (Saracens)
Kyle Eastmond (Bath Rugby)
Manusamoa Tuilagi (Leicester Tigers)
Billy Twelvetrees (Gloucester Rugby)
Christian Wade (London Wasps)
Marland Yarde (London Irish)
Ben Youngs (Leicester Tigers)
ENGLAND SAXONS EPS (32)
Calum Clark (Northampton Saints)
Jordan Crane (Leicester Tigers)
Paul Doran Jones (Harlequins)
Will Fraser (Saracens)
James Haskell (London Wasps)
Tom Johnson (Exeter Chiefs)
Graham Kitchener (Leicester Tigers)
George Kruis (Saracens)
Kearnan Myall (London Wasps)
David Paice (London Irish)
George Robson (Harlequins)
Ed Slater (Leicester Tigers)
Henry Thomas (Sale Sharks)
Thomas Waldrom (Leicester Tigers)
Luke Wallace (Harlequins)
Rob Webber (Bath Rugby)
Nick Wood (Gloucester Rugby)
Anthony Allen (Leicester Tigers)
Luther Burrell (Northampton Saints)
Elliot Daly (London Wasps)
George Ford (Bath Rugby)
Jonathan Joseph (Bath Rugby)
Jonny May (Gloucester Rugby)
Ugo Monye (Harlequins)
Stephen Myler (Northampton Saints)
Jack Nowell (Exeter Chiefs)
Charlie Sharples (Gloucester Rugby)
Joe Simpson (London Wasps)
David Strettle (Saracens)
Mathew Tait (Leicester Tigers)
Joel Tomkins (Saracens)
Richard Wigglesworth (Saracens)