Nokwe replaces the injured Bryan Habana, who has been ruled out with a hamstring injury. Free State winger Nokwe scored a try on debut two weeks ago in the Mandela Challenge match against Australia.
Jantjes returns in the place of Percy Montgomery, who celebrated his 100th cap in the loss against New Zealand last weekend.
South Africa team to face Australia in Durban on Saturday: Conrad Jantjes, JP Pietersen, Adrian Jacobs, Jean de Villiers, Jongi Nokwe, Butch James, Fourie du Preez, Tendai Mtawarira, Bismarck du Plessis, CJ van der Linde, Andries Bekker, Victor Matfield (capt), Schalk Burger, Juan Smith, Pierre Spies.
Reserves: Adriaan Strauss, Brian Mujati, Joe van Niekerk, Luke Watson, Ricky Januarie, Frans Steyn, Percy Montgomery.