Mazzucato believes Italy could punish England in the RBS 6 Nations clash should they take their eye off the ball.
He said: "I still believe they are strong favourites against us, but after the World Cup can be a dangerous time for the strongest team."
Mazzucato's expectations have also been raised by playing against Wasps in this season's European Cup.
New England captain Lawrence Dallaglio and full-back Josh Lewsey did not play in either game, and Mazzucato believes those involved Down Under may be feeling the strain of their exertions.
He added: "I played few games in the Heineken Cup and I saw few of them a little tired and this could be a big advantage for us.
"I do not say we can win this match, but we can take something very positive."
Before the clash in Rome's Stadio Flaminio, Italy will face a World XV side made up of foreigners playing in the Super 10.
Mazzucato is very excited by the inaugural event, and hopes to win a place in the starting line-up against the English with a strong performance.
He said: "I'm very happy to be part of the team which will challenge the World XV at Treviso and hopefully I will on the pitch against the world champions.
"I'm in good condition and I'm very much looking forward to playing in the Six Nations. It will be a wonderful dream for me if I get the chance to play against the reigning champions."