The Gran Parma star - capped 32 times by the Azzurri - broke his left leg in club action and has been ruled out of action for at least three months.
John Kirwan's Italy side start their RBS 6 Nations campaign against world champions England on February 15 in Rome.
"I'm very sad to not be part of the Italian national team, especially as I was in exceptional form," he told www.federugby.it, the FIR official website.
"I have waited for this opportunity for a long time and I believe I was very close to making the team. It is a big blow."