Winger Nicola Mazzucato is relegated to the bench for Masi, the 21-year-old from L'Aquila, had made two substitute appearances earlier in the tournament.
He reinforces a backs division still deprived of Mauro Bergamasco who has now missed his country's last three matches.
BergamascoÃ¢??s last trip to Murrayfield had been a memorable one with his 2001 solo try in Edinburgh voted the try of that year's tournament.
However, his younger brother Mirco will be taking the full-back berth, a role he has begun to make his own with successive tries in the matches against England and France.
Ramiro Pez, as expected, continues at stand-off in place of Diego Dominguez.
Italy team to play Scotland in RBS 6 Nations:
M Bergamasco (Padova); P Vaccari (Calvisano), A Masi (L'Aquila), G Raineri (Calvisano), D Dallan (Treviso), R Pez (Rotherham), A Troncon (Treviso, capt); A Lo Cicero (Lazio), C Festuccia (GRAN Parma), R Martinez (Treviso), C Bezzi (Viadana), M Giacheri (Rotherham), A De Rossi (Calvisano), A Persico (Viadana), M Phillips (Viadana)
Replacements: F Ongaro (Treviso), L Castrogiovanni (Calvisano), S Dellape (Treviso), S Palmer (Treviso), M Mazzantini (Rovigo), G Peens (Overmach Parma), N Mazzucato (Treviso)