Flutey, who played in six games during the Lions' 2009 tour of South Africa including the third-Test victory, is the biggest doubt for Martin Johnson's men after leaving the field with a calf injury.
Shaw, a Lions veteran with three tours under his belt, is also struggling with a calf problem that forced him to withdraw from the Aviva Premiership clash with Northampton.
Joe Simpson, a member of the second-string England Saxons outfit, is also a concern after being hampered by a shoulder injury.
"Riki will be assessed by England," said Wasps coach Tony Hanks.
"Hopefully he won't be out for too long.
"These things can take a day or two before you learn how bad they are, but he was definitely pretty sore.
"Simon will also be looked at by England. He had a calf strain on Friday. It was innocuous but he wasn't fit to play. Hopefully it's not long-term.
"Joe has a sore shoulder. He's being looked at. It was late in the game but, whether or not he could carry on, we were going to take him off."