The former Leeds coach had been widely tipped to turn to youth in his first squad selection since replacing Martin Johnson and he has done just that.
Saracens star Owen Farrell and his clubmate Brad Barritt are both included, as is fellow potential debutant Jordan Turner-Hall.
Scarlets No8 Ben Morgan, who choose England over Wales last week, Northampton trio Calum Clark, Phil Dowson and Lee Dickson, Quins prop Joe Marler and Wasps hooker Rob Webber are the other new faces also in contention to start against the Scots.
Mike Tindall, Mark Cueto and Nick Easter are all high-profile absentees, while fellow World Cup squad members Jonny Wilkinson, Lewis Moody and Steve Thompson all retired after the global gathering in New Zealand.
Shontayne Hape and 2009 Lions tourist Riki Flutey also failed to make the cut having been in the last Elite Player Squad.
Lancaster has named a further four players with just one cap apiece in Chris Robshaw, Mouritz Botha, Charlie Sharples and Joe Simpson but the ex-Saxons supremo has decided against naming a captain until after his first week of training in late January.
"Lots of players have been putting their hands up and there were some tough calls to make," said Lancaster.
"In line with the selection philosophy about starting a new era for English rugby, we have taken the chance to make changes and give players opportunities to be part of the journey towards the World Cup in 2015," said Lancaster.
"We are looking both at the present and just as importantly the future. We wanted to get the right balance in potential and experience and giving us the flexibility to play in different ways.
"We need to use the upcoming games to build the collective experience and we are confident we have got the right mix to take England forward into this Six Nations and beyond."
Four players from the Saxons squad will provide temporary injury cover with Bath second row Dave Atwood, Saracens utility back Alex Goode, Leicester lock Geoff Parling and Gloucester centre Henry Trinder covering for Louis Deacon, Toby Flood, Courtney Lawes and Manu Tuilagi.
There are just four Lions in the senior EPS squad and only one in the Saxons, with Tom Croft, Lee Mears, Matt Stevens and Charlie Hodgson making the main squad and Ugo Monye the second-string party.
England Elite Player Squad (32)
Mouritz Botha (Saracens)
Calum Clark (Northampton Saints)
Alex Corbisiero (London Irish)
Dan Cole (Leicester Tigers)
Tom Croft (Leicester Tigers)
Louis Deacon (Leicester Tigers)
Phil Dowson (Northampton Saints)
Dylan Hartley (Northampton Saints)
Courtney Lawes (Northampton Saints)
Joe Marler (Harlequins)
Lee Mears (Bath Rugby)
Ben Morgan (Scarlets)
Tom Palmer (Stade Francais)
Chris Robshaw (Harlequins)
Matt Stevens (Saracens)
Rob Webber (London Wasps)
David Wilson (Bath Rugby)
Tom Wood (Northampton Saints)
Chris Ashton (Northampton Saints)
Brad Barritt (Saracens)
Mike Brown (Harlequins)
Lee Dickson (Northampton Saints)
Owen Farrell (Saracens)
Toby Flood (Leicester Tigers)
Ben Foden (Northampton Saints)
Charlie Hodgson (Saracens)
Joe Simpson (London Wasps)
Charlie Sharples (Gloucester Rugby)
David Strettle (Saracens)
Manu Tuilagi (Leicester Tigers)
Jordan Turner-Hall (Harlequins)
Ben Youngs (Leicester Tigers)
Saxons squad (v Ireland Wolfhounds and Scotland A)
David Attwood (Bath Rugby)
Chris Brooker (Harlequins)
Paul Doran Jones (Northampton Saints)
Matt Garvey (London Irish)
James Gaskell (Sale Sharks)
Jamie Gibson (London Irish)
Joe Gray (Harlequins)
Tom Johnson (Exeter Chiefs)
Joe Launchbury (London Wasps)
Matt Mullan (Worcester Warriors)
Luke Narraway (Gloucester Rugby)
David Paice (London Irish)
Geoff Parling (Leicester Tigers)
George Robson (Harlequins)
Andrew Saull (Saracens)
Henry Thomas (Sale Sharks)
Thomas Waldrom (Leicester Tigers)
Nick Wood (Gloucester Rugby)
Nick Abendanon (Bath Rugby)
Anthony Allen (Leicester Tigers)
Delon Armitage (London Irish)
Matt Banahan (Bath Rugby)
Freddie Burns (Gloucester Rugby)
Karl Dickson (Harlequins)
Alex Goode (Saracens)
Jonathan Joseph (London Irish)
Ryan Lamb (Northampton Saints)
Jonny May (Gloucester Rugby)
Ugo Monye (Harlequins)
Henry Trinder (Gloucester Rugby)
Billy Twelvetrees (Leicester Tigers)
Micky Young (Leicester Tigers)
NB Bold denotes Lion