The Lions leave on May 25, and England fly-half Wilkinson could notch up another four appearances before then.
He made a successful return off the bench during the Falcons' narrow defeat at Northampton earlier this month, having been sidelined since suffering a knee injury against Harlequins five weeks earlier.
"He is fit and raring to go, and I know he can't wait to play in front of the Newcastle fans for the first time in a while," said Falcons rugby director Rob Andrew.
"There are no question marks over his fitness at all, and we look forward to him being part of a good team performance, hopefully followed by a wildcard semi-final and final."
It will be only Wilkinson's second Kingston Park appearance this year, as he looks to put a catalogue of post-World Cup injury setbacks behind him.