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With those men missing, Jones has opted to inject some new blood into his ranks with six forwards and nine backs heading to Argentina looking for a first international cap.
In the pack, props Will Collier of Harlequins and Harry Williams of Exeter Chiefs are included with Saracens second row Nick Isiekwe and back-row forwards Sam Underhill, as well as Sale Sharks' pair of brothers Ben and Tom Curry.
In the backs Exeter scrum-half Jack Maunder receives his first senior call-up alongside fly-half Piers Francis - who will join Northampton this summer - backs Alex Lozowski (Saracens) and Harry Mallinder of Northampton, Harlequins centre Joe Marchant, Sam James and Denny Solomona of Sale, Saracens' Nathan Earle and London Irish youngster Joe Cokanasiga.
Click here for the official 41-man Lions squad
The squad will assemble at The Lensbury on Sunday 29 May to begin their preparations for the two-Test tour which sees England play at Estadio San Juan del Bicentenario in San Juan on Saturday 10 June and Estadio Brigadier General Estanislao Lopez in Sante Fe, on Saturday 17 June.
And despite his new-look group, Jones is targeting a victorious trip to South America.
"We have picked the best squad we have available and we are looking forward to going to Argentina and winning 2-0," said Jones.
"The squad have a great opportunity to represent their country and win for England. There are a few new faces in the set-up so every training session and team meeting will count.
"The Pumas are a physically aggressive side and they have become very good at playing unstructured rugby; we need to make sure we control the structural part of the game and our defence will play an absolutely crucial role.
"Dylan Hartley will lead the side again having captained the team excellently during the RBS 6 Nations and will be supported by vice-captains Mike Brown, Danny Care and George Ford."
England's 31-man squad for Argentina tour
Will Collier (Harlequins, uncapped)
Luke Cowan-Dickie (Exeter Chiefs, 4 caps)
Ben Curry (Sale Sharks, uncapped)
Tom Curry (Sale Sharks, uncapped)
Charlie Ewels (Bath Rugby, 3 caps)
Ellis Genge (Leicester Tigers, 1 cap)
Dylan Hartley - captain (Northampton Saints, 84 caps)
James Haskell (Wasps, 75 caps)
Paul Hill (Northampton Saints, 5 caps)
Nathan Hughes (Wasps, 8 caps)
Nick Isiekwe (Saracens, uncapped)
Joe Launchbury (Wasps, 42 caps)
Matt Mullan (Wasps, 15 caps)
Chris Robshaw (Harlequins, 55 caps)
Sam Underhill (Ospreys/Bath Rugby, uncapped)
Harry Williams (Exeter Chiefs, uncapped)
Tom Wood (Northampton Saints, 50 caps)
Mike Brown - vice-captain (Harlequins, 60 caps)
Danny Care - vice captain (Harlequins, 71 caps)
Joe Cokanasiga (London Irish, uncapped)
Nathan Earle (Saracens, uncapped)
George Ford - vice captain (Bath Rugby, 35 caps)
Piers Francis (Auckland Blues/Northampton Saints, uncapped)
Sam James (Sale Sharks, uncapped)
Alex Lozowski (Saracens, uncapped)
Harry Mallinder (Northampton Saints, uncapped)
Joe Marchant (Harlequins, uncapped)
Jack Maunder (Exeter Chiefs, uncapped)
Jonny May (Gloucester Rugby, 25 caps)
Henry Slade (Exeter Chiefs, 4 caps)
Denny Solomona (Sale Sharks, uncapped)
England's two-Test tour to Argentina
Estadio San Juan del Bicentenario, San Juan, Kick-off 4.15pm (local time), Saturday 10 June
Estadio Brigadier General Estanislao Lopez, Santa Fe. Kick-off 4.15pm (local time), Saturday 17 June